How Customers Are Using Our qScript Ultra SuperMix

Here’s something you may not know: our customers are legit rock stars! We are impressed every day by the unexpected and creative ways they apply our tools and kits to accelerate their research. We are honored to contribute a small part toward the breakthroughs they make.

In this post, we’re sharing some papers and preprints that highlight the breadth of applications for which customers are deploying our qScript Ultra SuperMix, a highly stable next-generation SuperMix for first-strand cDNA synthesis. This product is faster and tougher than other options, delivering a 10-minute reaction time while supporting RT activity up to 65°C and overcoming challenging secondary structures.

Here are brief summaries of the studies and how this product was used.

Secretory carrier membrane proteins assist with aquaporin trafficking in Arabidopsis

First author: Qihang Jiang

Study description: Researchers took a deep dive into secretory carrier membrane proteins in Arabidopsis, suggesting a link between them and the plant’s drought tolerance phenotype.

They used qScript Ultra SuperMix to: perform reverse transcription of RNA from mutant and wild type plants.

Corticosteroids elevate intraocular pressure through suppression of TREK-1 signaling

First author: Sarah N. Redmon

Study description: Scientists researched the role of a potassium channel to regulate biological changes associated with ocular hypertension.

They used qScript Ultra SuperMix to: synthesize cDNA to analyze gene expression.

The Association Between Promoter Tandem Repeat Polymorphism (pVNTR) and CYP2C9 Gene Expression in Human Liver Samples

First author: Abelardo D. Montalvo

Study description: Researchers evaluated a variable number tandem repeat polymorphism found in the CYP2C9 gene to determine its role in gene expression and subsequent drug metabolism.

They used qScript Ultra SuperMix to: prepare cDNA with gene-specific primers and oligo(dT).

Next generation thiazolyl ketone inhibitors of cytosolic phospholipase A₂ α for targeted cancer therapy

First author: Felicity J. Ashcroft

Study description: Scientists tested in vitro and cellular activities of a new generation of thiazolyl ketone inhibitors, identifying a potent lead molecule that could one day be useful for acute leukemias and related cancers.

They used qScript Ultra SuperMix to: confirm gene knockdown by synthesizing cDNA from RNA samples.

Housing Temperature Impacts the Systemic and Tissue-Specific Molecular Responses to Cancer in Mice

First author: Andrea Irazoki

Study description: Using a mouse model, researchers sought to understand how temperature-induced stress might reveal molecular mechanisms associated with the loss of muscle and fat commonly seen in patients with cancer.

They used qScript Ultra SuperMix to: perform reverse transcription on RNA for gene expression analysis.

Human ocular fluid outflow on-chip reveals trabecular meshwork-mediated Schlemm’s canal endothelial dysfunction in steroid-induced glaucoma

First author: Renhao Lu

Study description: Scientists built an on-chip model to study human ocular fluid outflow, a key contributor in the development of glaucoma.

They used qScript Ultra SuperMix to: perform reverse transcription of RNA collected from cells in the coculture system.

Congratulations to all of these scientists and the rest of our customers doing cool work with this product! To learn more about how it’s being used, click on the citations badge in the left-hand column on this page.