There’s nothing we like more than helping our customers generate better, more reliable research results. But one thing comes close: perusing the scientific literature to learn about all the different ways scientists are making use of our products. (Don’t tell the boss, but this is our favorite way to procrastinate. Even better than cat videos.)
Today we’re sharing a sampling of recent preprints and publications that feature our repliQa HiFi ToughMix, a 2x, ready-to-use master mix that contains all the components for high-fidelity PCR amplification, including a genetically modified DNA polymerase coupled with hot start antibodies. This next-generation master mix provides 90-fold higher fidelity compared to Taq, while reducing time to PCR results by two to three times.
First author: Christopher B. Forsyth
Study description: Researchers studied fecal microbiota to determine whether changes in the expression of a certain gene were associated with aging-related inflammation.
They used repliQa HiFi ToughMix to: analyze DNA extracted from mice fecal pellets.
First author: Olga Kosewska
Study description: Scientists studied various cultivars of wheat and barley to understand their impact on the microbiome of beetles that are known pests of stored cereals.
They used repliQa HiFi ToughMix to: perform bacterial 16S rRNA amplification of material from the beetles’ digestive tracts.
First author: Klemen Mezgec
Study description: Researchers used modular protein engineering to build rigid rods that were several micrometers long; these could be used in the future as scaffolding for other proteins.
They used repliQa HiFi ToughMix to: amplify the vector backbone for the Gibson assembly reaction.
First author: Khaled Aboushaala
Study description: Scientists studied the state of the gut microbiome in people with and without lumbar degenerative spondylolisthesis, a condition leading to degeneration in the spine.
They used repliQa HiFi ToughMix to: perform two PCR amplifications of the genomic DNA extracted from stool samples submitted by study participants.
First author: Maya Moshe
Study description: Researchers evaluated a potential pesticide alternative, studying bacterial biocontrol agents and whether they could prevent disease in plants despite the presence of a fungal pathogen.
They used repliQa HiFi ToughMix to: perform two rounds of PCR to amplify 16S rRNA gene content from bacteria and internal transcribed spacer regions from the fungal pathogen.
First author: R. Forman
Study description: The team studied animals that had been reintroduced in a region of Israel to determine whether their presence had any implications in disease trends, either to the reintroduced animals or to the local wildlife.
They used repliQa HiFi ToughMix to: perform PCR amplification of DNA extracted from fecal samples of wild asses.
First author: Serhii Filatov
Study description: Scientists characterized tick populations across Ukraine, Georgia, and Azerbaijan, sequencing and analyzing mitochondrial genomes to better understand ticks that transmit diseases in this area.
They used repliQa HiFi ToughMix to: perform long-range PCR reactions of complete mitochondrial DNA collected from 54 Ornithodoros verrucosus ticks.
First author: Mary Nkongho Tanyitiku
Study description: Researchers used a model system to explore whether land snails harboring E. coli could be a vector of transmission to people who eat them.
They used repliQa HiFi ToughMix to: confirm the presence of Shigatoxigenic genes in E. coli strains during the study.
First author: Chetan C. Gaonkar
Study description: This team evaluated the use of long-read nanopore sequencing to characterize the 18S rDNA regions used in protist diversity studies.
They used repliQa HiFi ToughMix to: Perform amplification of genomic DNA for the major protist groups.
First author: Joseph D. Berleant
Study description: Scientists used protective microcapsules together with combinatorial DNA barcodes to pool 96 mock viral genome samples as a means of evaluating the ability to achieve scalable storage and retrieval of samples.
They used repliQa HiFi ToughMix to: amplify samples prior to qPCR.
To learn more about how customers are using this product, click on the citations badge in the left-hand column on this page.